NE3A X-ray Beamline for High-throughput Macromolecular Crystallography
NE3A is deigned for high-throughput macromolecular crystallography which is applied in pharmaceutical researches. Using double crystal monochromator cooled with liquid nitrogen, a wide energy range (6.5 - 17 keV) of the the monochromatic beam is available with fine energy resolution. A high-precision (SOC is less than 2 micron) sample axis allows experiments with micro-crystals. A robot for sample exchange will be installed to improve the efficiency of experiments.
SCHEMATIC VIEW OF THE BEAMLINE
Area of Research
Macromolecular crystallography
Light Source
In-vacuum Undulator (with taper option)
Optics
Collimating Mirror: 19.2 m
Rhodium coated silicon single crystal (Flat shaped, 1000 mm (L) x 80 mm (W) x 70 mm (T) ) with 3.5 mrad glancing angle.
Mono-chromator: 20.8 m
Numerical link type Si(111) double crystal monochromator, liquid nitrogen cooling
Focusing Mirror: 22.9 m
Rhodium coated SiO2 (Troidal, 1000 mm (L) x 100 mm (W) x 65 mm (T) ) with 3.5 mrad glancing angle.
Photons at Sample Position
Energy range : 0.70 - 1.9 Å
Energy resolution : 2 x 10-4
Photon flux : 8.0 x 1011@6.5 GeV, 40 mA, 1.0 Å
(0.2 x 0.2 mm slit)
Facilities in the Experimental Station
Detector : ADSC Quantum 270
Detector size : 270 x 270 mm
Camera distance : 60 - 500 mm
Vertical offset : 0 - 150 mm
Cryocooling device : nitrogen gas flow around 95 K
Fluorescence detector : Si-PIN photodiode

Workstation and Softwares
Computers
4 PC Workstations (Linux, CentOS 5.2)
2 PC Windows (USB2.0, IEEE1394)
Softwares for image processing
HKL2000, DPS/Mosflm/Scala, XDS
Reference
to be published...
Contact Person
Yusuke Yamada (KEK-PF)
Phone : +81-298-64-5642
Fax : +81-298-64-2801
e-mail : yusuke.yamada@kek.jp
Other Information
Current photograph of NE3A
Comparison with other PX beamlines in PF
User Manual for PX beamlines
Brief information and guide to use PF-AR NE3A
PX beamlines at PF Japanese/ English
Beamtime schedules, beamline news, and so on
Structural Biology Research Center

Last modified: 2009-02-12