BL-1A: Protein Micro-Crystallography and Low-Energy SAD Experiment Beamline
BL-1A is dedicated to microcrystallography and long wavelength SAD (Single Anomalous dispersion) experiments. The beamline is designed to take full advantage of a long wavelengh X-ray beam at around 3 A to further enhance anomalous signals. The light source is an in-vacuum short gap undulator optimized at around the wavelength with the fundamental harmonics to obtain maximum brilliance. The vacuum section of the beamline has only one terminal beryllium window, followed by a diffractometer equipped with a helium cryostream and a specially designed helium chamber to minimize the attenuation of the lower energy beam and background noises. A cryo-cooled channel-cut monochromator and bimorph KB focusing mirrors compose a simple optics to deliver a focused beam with a good stability.
SCHEMATIC VIEW OF THE BEAMLINE
Area of Research
Macromolecular crystallography
Light Source
Short Gap Undulator
Periodic length : 12 mm
Periodic Number : 39
Maximum K value : 0.78
Optics
Mono-chromator 16.5 m
Cryo-cooled channel-cut Si(111)
Vertical Focusing Mirror 18.6 m
16 electrodes bimporh (600 mm (L) x 45 mm (W)), Rh/SiO2 coated with 4.0 mrad glancing angle.
Horizontal Focusing Mirror 19.2 m
12 electroldes bimporh (450 mm (L) x 45 mm (W)), two stripes (Rh/SiO2) with 4.0 mrad glancing angle.
Photons at Sample Position
Energy range : 0.95 - 1.1 Å, 2.7 - 3.3 Å
Energy resolution : 2 x 10-4
Photon flux : 4 x 1011, 450 mA, 1.1 Å
2 x 1011, 450 mA, 3.3 Å
(beam size: 0.03x0.01 mm)
Facilities in the Experimental Station
Detector: Dectris PILATUS 2M-F
Detector size : 253 x 289 mm
Camera distance : 50 - 460 mm
Vertical offset : 0 - 150 mm
Cryocooling device : CryoIndusty Co. G2B-LT
(nitrogen gas flow around 95 K, helium gas flow 30 - 95 K)
Fluorescence detector : SSD
Workstation and Softwares
Computers:
5 Linux (CentOS6)
2 Windows ( USB2.0 )
Software:
HKL2000, DPS/Mosflm/Scala, XDS
Reference
Contact Person
Naohiro Matsugaki (KEK-PF)
Phone : +81-298-64-5647
Fax : +81-298-64-2801
e-mail : naohiro.matsugaki@kek.jp
Other Information
Current photographs of BL-1A
Comparison with other PX beamlines in PF
Preliminary test experiments at BL-1A
Notes for data process of PILATUS 2M data
PX beamlines at PF Japanese/ English
Structural Biology Research Center
Last modified: 2012-09-03