BL-17A: Protein Micro-Crystallography and Low-Energy SAD Experiment Beamline
BL-17A is a new beam line for protein crystallographic experiments at the Photon Factory. The high brilliance beam derived from the new short-gap undulator, SGU#17 offers unique possibilities for protein crystallographic experiments at the Photon Factory including micro-crystal structure analysis and structure determination using softer Xrays. The extremely small beam size of the short-gap undulator source, together with advances in X-ray optics, allows outstanding performance at modest cost even with 2nd generation synchrotron facility.
The main optical elements are the double crystal monochromator and the K-B mirror system for fine focusing. The monochromator consists of flat Si(111) crystals which are cooled with liquid nitrogen. The K-B mirror system after the monochromator focuses the synchrotron beam on the sample position. It consists of a flat-bent mirror for vertical focusing and an elliptical-bent mirror for horizontal focusing which is achieved by the gArm Methodh mirror bender. The BL-17A delivered the first beam in October, 2005. After beam diagnostics, alignment and installation of experimental apparatus, the beam line was opened for public users in 2006.
We installed a new high-speed and high-gain detector, ADSC Quantum 270 in 2007.
SCHEMATIC VIEW OF THE BEAMLINE
Area of Research
Macromolecular crystallography
Light Source
Short Gap Undulator
@@ Periodic length : 16 mm
@@ Periodic Number : 29
@@ Maximum K value : 1.27
Optics
Mono-chromator 17.5 m
Numerical link type Si(111) double crystal monochromator, liquid nitrogen cooling
Vertical Focusing Mirror 22.22 m
Rhodium coated silicon single crystal (Flat shaped, 1000 mm (L) x 100 mm (W) x 50 mm (T) ) with 4.0 mrad glancing angle.
Horizontal Focusing Mirror 29.5 m
Rhodium coated silicon single crystal (Flat shaped, 1000 mm (L) x 100 mm (W) x 40 mm (T) ) with 4.0 mrad glancing angle.
Photons at Sample Position
(at 2.5 GeV operation)
Energy range : 0.95 - 1.33 Å, 1.8 - 2.2 Å
(Energy range at 3.0 GeV : 1.0 - 1.55 Å)
Energy resolution : 2 x 10-4
Photon flux : 6.6 x 109, 400 mA, 1.0 Å
1.3 x 1010, 400 mA, 2.0 Å
(0.02x0.02 mm slit)
Facilities in the Experimental Station
Detector: ADSC Quantum 270 (cf. data process)
Detector size : 270 x 270 mm
Camera distance : 40 - 700 mm
Vertical offset : 0 - 100 mm
Cryocooling device : RIGAKU cryo systems
@@@@@@@@@ (nitrogen gas flow around 95 K)
Fluorescence detector : SSD
Workstation and Softwares
Computers
@@ 4 PC Workstations (Linux, CentOS5)
@@ 2 PC Windows ( USB2.0, IEEE1394)
Software
@@ HKL2000, DPS/Mosflm/Scala, XDS
Reference
N. Igarashi, N. Matsugaki, Y. Yamada, M. Hiraki, A. Koyama, K. Hirano, T. Miyoshi and S. Wakatsuki, AIP Conf. Proc., 879, 812-815 (2007)
N. Igarashi, K. Ikuta, T. Miyoshi, N. Matsugaki, Y. Yamada, MS. Yousef and S. Wakatsuki, J. Synch. Rad., 15, 292-295 (2008)
Contact Person
Noriyuki IGARASHI (KEK-PF)
Phone : +81-298-64-5641
Fax : +81-298-64-2801
e-mail : noriyuki.igarashi@kek.jp
Other Information
Current photographs of BL-17A
Comparison with other PX beamlines in PF
Preliminary test experiments at BL-17A
Notes for data process of Q270 data
PX beamlines at PF Japanese/ English
Structural Biology Research Center
Last modified: 2009-04-08