Overview of the experiment at the PFMX beamlines

Before starting the experiment

  1. Declaration of introduction of samples / chemicals
    Place the document called "Declaration of introduction of samples / chemicals" on the white board near the beamline you are experimenting in. To experiment without this document is strictly forbidden in either PF or PF-AR.

  2. Refueling of the liquid-nitrogen
    Whenever you need liqN2 for your experiments, please use the 100 L tank to fill in the smaller dewar. => more details

  3. Logging-in to the beamline computers
    You will need to Log-in to the computers for processes such as beamline control (UGUI), data analysis (PC1, 2...), and data download (User1, 2...). This can be done with a given ID and password.

  4. Start-up the beamline control software
    Start-up the beamline control software either by double-clicking on the icon called UGUI(S) on your Desktop, or by typing ugui (or eventually uguis) in terminal window on the beamline controlling computer (so-called UGUI computer).
    % ugui
    => more details

  5. Quick and rough adjustment of the goniometer position
    In the event where your cryo-pin has a length different from the one used by the previous user, your crystals might be out of the nitrogen gas flow. It is therefore highly recommended that you carry out one quick loop centering with your empty cryo-pin before starting your actual experiments with real crystal samples.

    Automatic distance adjustment between the sample and the cryo nozzle
    While in sample exchange and centering modes, the distance between the sample and the cryo nozzle is approximately 12.5 mm. This distance will automatically change at the time of EXAFS and diffraction data measurements, to approximate 5.5 mm. If you wish to have a different distance for the cryo nozzle, please contact the responsible staff (PHS 2613). As a general note, this might be necessary for capillary experiments. Therefore, if you wish to change the distance, we will respond as quickly as possible.

  6. Data Back-up
    You can back-up your data by connecting your own Hard Disk Drive to the Windows PC prepared for this purpose. For more information, please go to the => details

Diffraction experiments

  1. Crystal mounting
    1. Manual mounting.=> details
    2. PAM robot mounting.=> detalis (Japanese)

  2. Setting up the Working Directory
    Click on the [Select] button located at the top of the UGUI, create your working directory, and select it. All your experimental data will be stored into the selected directory.

  3. Crystal centering
    Adjust the sample position at the center of the goniometer rotation.

  4. Estimation of the sample quality and decision of the experimental protocol
    You can check the sample condition and quality by recording a snapshot of the crystal. Then, you can decide of the settings for further measurements (e.g. Start angle, Oscilation angle, Exporsure time, ...).

  5. Decision making of the wavelength for anomalous dispersion (EXAFS)
    Just start the program called EXAFS, that will display the wavelengths necessary for MAD data collections.

  6. Data collection of diffraction images
    You can start the data collection after recording either a snapshot or an EXAFS.

  7. Processing and analyzing the diffraction images
    Data processing and analysis can be performed on the Linux computers provided for each beamline (labeled PC1, PC2...). The list of processing and analysis softwares is available here.

  8. You can return to 1 when the next experiment is ready.

After the experiment

  1. Check your data backup!
    Please confirm that all your experimental data are in your back-up Hard Disk Drive before leaving the beamline. Please note that the crude data will stay on our server for several days only.

  2. Logout
    Please remember to log-out all the computers present at the beamline. (UGUI, PC1, 2, ..., User1, 2, ...,)

  3. Exhibition and collection of documents
    1. Complete the "Beamtime Report" =>Web form
    2. Collect your "Declaration of introduction of samples / chemicals" from the white board.

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