For BL-1A users

  1. We started the operation of GPFS.
    The default Working Directory is /gpfs/data/(UserID), as indicated in the UGUI.

  2. Do not close the BBS during your experiments.
    Close the experimental hutch and keep the shutter open if you don't perform experiments for a long time or if you finish your experiments.

  3. Please close the GAP after every injection.
    • Click the [Gap] button in the Special menu of the beamline control software UGUI.



    • After appearance of the confirmation widget on the UGUI screen, the gap will be closed to a value corresponding to the current wavelength (specified within the UGUI).



Specifications concerning BL-1A

Area of Research

Macromolecular Crystallography

Light Source

Short Gap Undulator

Optics

Monochromator
16.5 m
Cryo-cooled channel-cut Si(111)
Vertical
Focusing Mirror
18.6 m
16 electrodes bimporh (600 mm (L) x 45 mm (W)),
Rh/SiO2 coated with 4.0 mrad glancing angle.
Horizontal
Focusing Mirror
19.2 m
12 electroldes bimporh (450 mm (L) x 45 mm (W)),
two stripes (Rh/SiO2) with 4.0 mrad glancing angle.

Photons at Sample Position

Energy range : 0.95 - 1.1 Å, 2.7-3.3 Å
Energy resolution (deltaE / E) : 2.0 x 10-4
Photon Flux : 4.0 x 1011@2.5GeV, 450mA, 1.1 Å
                      2.0 x 1011@2.5GeV, 450mA, 3.3 Å
Standard slit size (H x V) : 0.03 x 0.01 mm

Facilities in the Experimental Station

X-ray Detector : Eiger X4M
Type : PAD
Detector size : 155.2 x 162.5 mm
Pixel size : 75 x 75 µm
Pixel number : 2070 x 2167
Frame data size : 2 MByte
Readout time : 0.00003 sec.
Detector distance : 30 - 450 mm
Cryocooling Device : CryoIndusty Co. G2B-LT
(nitrogen gas flow around 95 K, helium gas flow 30 ~ 95 K)
Fluorescence Detector : Si-PIN diode

Workstation and Softwares

Computers
   5 PC Workstations ( Linux CentOS6 )
   2 PC Windows ( WindowsXP USB2.0 )

Available software for Image Processing : HKL2000, iMosflm, XDS

References

Improvement of an Automated Protein Crystal Exchange System PAM for High-throughput Data Collection
Masahiko Hiraki, Yusuke Yamada, Leonard M. G. Chavas, Soichi Wakatsuki, Naohiro Matsugaki
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795550/

Data Management System at the Photon Factory Macromolecular Crystallography Beamline
Yusuke Yamada, Naohiro Matsugaki, Leonard M. G. Chavas, Masahiko Hiraki, Noriyuki Igarashi, Soichi Wakatsuki, Journal of Physics: Conference Series ; 425, 012017 (2013)
http://iopscience.iop.org/1742-6596/425/1/012017

Current stats and future prospects of an automated sample exchange system PAM for protein crystallography
Masahiko Hiraki, Yusuke Yamada, Leonard M. G. Chavas, Naohiro Matsugaki, Noriyuki Igarashi, Soichi Wakatsuki, Journal of Physics: Conference Series ; 425, 012014 (2013)
http://iopscience.iop.org/1742-6596/425/1/012014

Fully Automated Data Collection Using PAM and the Development of PAM/SPACE Reversible Cassettes
Masahiko Hiraki, Shokei Watanabe, Leonard M. G. Chavas, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Soichi Wakatsuki, Masahiro Fujihashi, Kunio Miki, Seiki Baba, Go Ueno, Masaki Yamamoto, Mamoru Suzuki, Atsushi Nakagawa, Nobuhisa Watanabe, and Isao Tanaka, AIP Conference Proceedings, 1234, 673 (2010)
http://proceedings.aip.org/resource/2/apcpcs/1234/1/673_1

High-throughput operation of sample-exchange robots with double tongs at the Photon Factory beamlines
Masahiko Hiraki, Shokei Watanabe, Nobuo pHonda, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Yurii Gaponov, Soichi Wakatsuki, Journal of Synchrotron Radiation, Vol.15, 300-303 (2008)
http://www.ncbi.nlm.nih.gov/pubmed/18421164

Implementation of remote monitoring and diffraction evaluation systems at the Photon Factory macromolecular crystallography beamlines
Yusuke Yamada, Nobuo pHonda, Naohiro Matsugaki, Noriyuki Igarashi, Masahiko Hiraki, Soichi Wakatsuki, Journal of Synchrotron Radiation, Vol.15, 296-299,(2008)
http://www.ncbi.nlm.nih.gov/pubmed/18421163

Automated Sample Exchange Robots for the Structural Biology Beam Lines at the Photon Factory
Masahiko Hiraki, Shokei Watanabe, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Yurii Gaponov, Soichi Wakatsuki, AIP Conference Proceedings, 879, 1924 (2007)
http://proceedings.aip.org/resource/2/apcpcs/879/1/1924_1

Automation in Structural Biology Beamlines of the Photon Factory
Noriyuki Igarashi, Masahiko Hiraki, Naohiro Matsugaki, Yusuke Yamada, Soichi Wakatsuki, Journal of the Crystallographic Society of Japan, Vol.49(6), 359-363 (2007)
https://www.jstage.jst.go.jp/article/jcrsj1959/49/6/49_6_359/_article/-char/ja/

Contact Person

Naohiro Matsugaki (KEK-PF)
Phone : +81-29-864-5647
PHS : +81-29-864-5200 (ext : 4340)
FAX : +81-29-864-2801
E-mail : naohiro.matsugakikek.jp


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