For BL-5A users

  1. We started the operation of GPFS.
    The default Working Directory is /gpfs/data/(UserID), as indicated in the UGUI.

  2. Do not close the BBS during your experiments.
    Close the experimental hutch and keep the shutter open if you don't perform experiments for a long time or if you finish your experiments.


Specifications concerning BL-5A

Area of Research

Macromolecular Crystallography

Light Source

Multipole Wiggler

Optics

Collimating Mirror
14.5 m
Rhodium coated silicon single crystal
Glancing angle : 3.5 mrad
(Flat shaped, 1000 mm (L) x 80 mm (W) x 70 mm (T) )
Monochromator
16.5 m
Numerical link type Si(111) double crystal monochromator, direct water cooling
(1st crystal : Micro-channel water cooling)
(2nd crystal : Indirect water cooling)
Focusing Mirror
18.7 m
Rhodium coated silicon single crystal
Glancing angle : 3.5 mrad
(Troidal, 1000 mm (L) x 100 mm (W) x 60 mm (T) )

Photons at Sample Position

Energy range : 0.75 - 1.9 Å
Energy resolution (deltaE / E) : 2.5 x 10-4
Photon Flux : 8.6 x 1011@2.5GeV, 450mA, 1.0 Å
Standard slit size (H x V) : 0.2 x 0.2 mm

Facilities in the Experimental Station

X-ray Detector : ADSC Quantum 315r
Type : CCD
Detector size : 315 x 315 mm
Pixel size : 102 x 102 µm
     (Binning off : 51 x 51 µm)
Pixel number : 3072 x 3072
     (Binning off : 6144 x 6144)
Frame data size : 19 MByte
Readout time : 0.25 秒
Time for collecting a frame : Exp. time + 1.3秒
Detector distance : 60 - 950 mm (up to 950 mm on demand)
Detector vertical offset : 0 - 150 mm
Cryocooling Device : Nitrogen gas flow (95 K ~ 300 K)
Fluorescence Detector : Si-PIN diode

Workstation and Softwares

Computers
   4 PC Workstations ( Linux CentOS6 )
   2 PC Windows ( WindowsXP USB2.0 )

Available software for Image Processing : HKL2000, iMosflm, XDS

References

Improvement of an Automated Protein Crystal Exchange System PAM for High-throughput Data Collection
Masahiko Hiraki, Yusuke Yamada, Leonard M. G. Chavas, Soichi Wakatsuki, Naohiro Matsugaki
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3795550/

Data Management System at the Photon Factory Macromolecular Crystallography Beamline
Yusuke Yamada, Naohiro Matsugaki, Leonard M. G. Chavas, Masahiko Hiraki, Noriyuki Igarashi, Soichi Wakatsuki, Journal of Physics: Conference Series ; 425, 012017 (2013)
http://iopscience.iop.org/1742-6596/425/1/012017

Current stats and future prospects of an automated sample exchange system PAM for protein crystallography
Masahiko Hiraki, Yusuke Yamada, Leonard M. G. Chavas, Naohiro Matsugaki, Noriyuki Igarashi, Soichi Wakatsuki, Journal of Physics: Conference Series ; 425, 012014 (2013)
http://iopscience.iop.org/1742-6596/425/1/012014

UV LED lighting for automated crystal centring
Leonard M. G. Chavas, Yusuke Yamada, Masahiko Hiraki, Noriyuki Igarashi, Naohiro Matsugaki, Soichi Wakatsuki, Journal of Synchrotron Radiation, 18, 11-15 (2011)
http://www.ncbi.nlm.nih.gov/pubmed/21169682

Fully Automated Data Collection Using PAM and the Development of PAM/SPACE Reversible Cassettes
Masahiko Hiraki, Shokei Watanabe, Leonard M. G. Chavas, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Soichi Wakatsuki, Masahiro Fujihashi, Kunio Miki, Seiki Baba, Go Ueno, Masaki Yamamoto, Mamoru Suzuki, Atsushi Nakagawa, Nobuhisa Watanabe, and Isao Tanaka, AIP Conference Proceedings, 1234, 673 (2010)
http://proceedings.aip.org/resource/2/apcpcs/1234/1/673_1

High-throughput operation of sample-exchange robots with double tongs at the Photon Factory beamlines
Masahiko Hiraki, Shokei Watanabe, Nobuo pHonda, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Yurii Gaponov and Soichi Wakatsuki, Journal of Synchrotron Radiation, Vol.15, 300-303 (2008)
http://www.ncbi.nlm.nih.gov/pubmed/18421164

Implementation of remote monitoring and diffraction evaluation systems at the Photon Factory macromolecular crystallography beamlines
Yusuke Yamada, Nobuo pHonda, Naohiro Matsugaki, Noriyuki Igarashi, Masahiko Hiraki, Soichi Wakatsuki, Journal of Synchrotron Radiation, Vol.15, 296-299,(2008)
http://www.ncbi.nlm.nih.gov/pubmed/18421163

Automated Sample Exchange Robots for the Structural Biology Beam Lines at the Photon Factory
Masahiko Hiraki, Shokei Watanabe, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Yurii Gaponov, and Soichi Wakatsuki, AIP Conference Proceedings, 879, 1924 (2007)
http://proceedings.aip.org/resource/2/apcpcs/879/1/1924_1

Automation in Structural Biology Beamlines of the Photon Factory
Noriyuki Igarashi, Masahiko Hiraki, Naohiro Matsugaki, Yusuke Yamada, Soichi Wakatsuki, Journal of the Crystallographic Society of Japan, Vol.49(6), 359-363 (2007)
https://www.jstage.jst.go.jp/article/jcrsj1959/49/6/49_6_359/_article/-char/ja/

Contact Person

Naohiro Matsugaki (KEK-PF)
Phone : +81-29-864-5647
PHS : +81-29-864-5200 (ext : 4340)
FAX : +81-29-864-2801
E-mail : naohiro.matsugakikek.jp


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